FRET detection of lymphocyte function–associated antigen-1 conformational extension

FRET detection of lymphocyte function–associated antigen-1 conformational extension

Lymphocyte function-associated antigen 1 (LFA-1, CD11a/CD18, αLβ2-integrin) and its ligands are essential for adhesion between T-cells and antigen-presenting cells, formation of the immunological synapse, and other immune cell interactions. LFA-1 function is regulated through conformational changes that include the modulation of ligand binding affinity and molecular extension. However, the rela...

Activation-induced conformational changes in the I domain region of lymphocyte function-associated antigen 1.

Conformational changes in integrins are important for efficient ligand binding during activation. We proposed that the I domain of the integrin lymphocyte function-associated antigen 1 (LFA-1) could exist in both open and closed conformations and generated constitutively activated LFA-1 by locking the I domain in the open conformation. Here we provide structural and biochemical evidence to vali...

Frequency of Bovine Lymphocyte Antigen DRB3.2 Alleles in Sarabi Cows

Detection of Cytotoxic T-Lymphocyte Associated Antigen-4 Gene Polymorphism in Type 1 Diabetes Mellitus.

Type 1 diabetes is one of the most common chronic childhood illnesses. Interplay between genetic susceptibility and environmental factors is thought to provide the fundamental element for the disease. It has been shown that more than 40 genetic loci are associated with T1DM. Important one among these is the CTLA-4. This work aimed to detect Cytotoxic T Lymphocyte-associated antigen 4 (CTLA-4) g...

BHV-1 Antigen Detection in Paraffinized Lung Sections of Pneumonic Sheep Lung Using Immunohistochemistry

Background: Respiratory tract infections caused by some viruses with cattle origin have been demonstrated in sheep and goats. OBJECTIVES: The main goal of this study was to determine Bovine Herpes virus type 1 BHV1antigen in formalin-fixed paraffin-embedded lung tissue of pneumonic sheep, using immunohistochemistry (IHC) staining method. METHODS: For this purpose, the lungs of 4079 sheep, which...